REFERENCE LIST OF LABORATORY TESTS CABINET FOR HEALTH SERVICES DEPARTMENT FOR PUBLIC HEALTH DIVISION OF LABORATORY SERVICES

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

SHIGELLA	Isolation Identification (referred culture)	Feces, urine, rectal swabs Rectal Swabs Food Agar slant: Heart infustion, trypticase soy, blood or choco-late agar slant.	#219 #219 #504 #219	Enteric pathogens kit with buffered glycerol saline. Special kit with Cary-Blair preservative and cold packs. Must be sent refrigerated. DO NOT FREEZE. Mark slip “Shigella”. Refer to Manual for Environmentalists and Milk Inspectors Mailing container furnished by submitter.	Bacteriology LAB 353
SICKLE CELL (infant)	Electrophoresis	Heel-stick blood applied to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional information, see back of form.	Newborn Screening
SICKLE CELL (children & adults)	Electrophoresis	Fingerstick blood applied to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional information, see back of form.	Newborn Screening
SPOROTRICHOSIS	Serology	5 ml clotted blood or serum	#213	Serology container.	Serology
STAPHYLOCOCCUS AUREUS	Isolation	Feces, vomitus Nasal swabs, skin, skin lesions Food	#219 #219 #504	Enteric pathogens kit with buffered glycerol saline. Only by request of the Division of Epidemiology. Swabs in sterile tubes. Only by request of the Division of Epidemiology. Refer to Manual for Environmentalists and Milk Inspectors. Only by request of the Division of Epidemiology for foodbourne disease outbreaks.	Bacteriology LAB 353
DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

SYPHILIS Note:Screening and confirmation tests performed according to DLS established algarithm	VDRL Qualitative & Quantitative	Clotted blood, serum, or spinal fluid. Serum	#213	Syphilis serology kit.	Serology LAB 351
TAPEWORM	Macroscopic & microscopic exam, formalin Hemo-D concentrations.	Feces and/or worm. For feces, submit at least three specimens on alternate days.	#219	Intestinal parasites, single vial container (10% formalin); Sterile jar with 10% formalin or 70% ethyl alcohol for the worm.	Bacteriology LAB 349A
TRICHINOSIS	Microscopic exam Bentonite flocculation	Biopsy of muscle (for food, see “Manual for the Sanitarian”) 5ml whole blood or serum	#219 #213	Intestinal parasites single vial container (10% formalin) Serology container	Bacteriology LAB 349A
TRIGLYCERIDE		See Lipid Profile
TUBERCULOSIS	Microscopic exam, culture identification by DNA Probe or HPLC, drug susceptibility studies.	Sputum, gastric wash, spinal fluid, pleural fluid, pus, urine, other body fluids. Gastric wash must be neutralized before mailing. Referred culture for identification and drug suscept-ibility studies.	#207	TB specimen kit Mailing container furnished by submitter.	Mycobacteria Lab LAB 355
TULAREMIA Note: Call the Division of Epidemiology or the Division of Lab Service for specific instructions	Agglutination test Isolation Identification (Referred culture)	Acute and convalescent sera. Chocolate agar slant.	#213 #219	Serology container. Mailing container furnished by submitter.	Bacteriology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

TYPHOID SALMONELLA SEROTYPE TYPHI	Culture Isolation Antigenic typing	Feces, urine Feces, urine, rectal swab Rectal swabs Culture agar or slant without sugar.	#219 #219 #219	Enteric pathogens kit. Enteric pathogens basic kit with buffered glycerol saline. Enteric pathogens special kit with Cary-Blair preservative. Must be refrigerated. DO NOT FREEZE. Mark slip “Salmonella typhi”. Mailing container furnished by submitter.	Bacteriology LAB 353
UNDULANT FEVER		See Brucellosis
VARICELLA ZOSTER		See Chickenpox
VDRL		See Syphilis
VIBRIO CHOLERAE AND OTHER VIBRIO SPECIES	Isolation Identification (referred culture)	Feces, urine, rectal swabs Agar slant: heart infusion, trypticase soy, chocolate or blood agar slant.	#219 #219	Enteric pathogens special kit with Cary-Blair preservative. Must be refrigerated. DO NOT FREEZE. Mark slip “Vibrio”. Mailing container furnished by submitter.	Bacteriology LAB 353

NOTE: For bacteriological and Chemical water analyses, all samples must be collected and submitted by authorized collectors. All samples from private sources must be received within 48 hours of collection. All samples from public drinking water systems must be received within 30 hours of collection. If additional tests are needed, contact the Division of Laboratory Services.
DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

WATER: BACTERIO-LOGICAL ANALYSES	Fecal coliform count & total coliform count. Heterotrophic plate count Pseudomonas Total coliform count and E. coli count. Presence or absence of total coliforms & E. coli.	Recreational water. Recreational water. Water from spas. Drinking water. Water from dairies.	#507 #507 #507 #507	Refer to the Manual for Environmentalists & Milk Inspectors for all tests.	Sanitary Bacteriology LAB 507E
WATER: Chemical ANALYSES	Alkalinity Calcium Chloride Color Fluoride Hardness Iron Magnesium Manganese Nitrates Odor PH Solids Sulfate Turbidity Dental Fluoride Metals Pesticides Petroleum Products	Drinking water from private supplies.	#700b #505c #504 #504 #504	Chemical analysis cubitainer. Call CHS-DPH Maternal and Child Health. Metal Cubitainer Call the lab. Call the lab.	Instrumentation Chemistry
WHOOPING COUGH		See Bordetella
YERSINIA PESTIS (PLAGUE)	NOTE: Call the Division of Epidemiology or the Division of Laboratory Services for specific instructions before collecting any specimens.

DISEASE/

CONDITION

TEST

SPECIMEN

LAB FORM

CONTAINER

SECTION AND LABEL

YERSINIA SPECIES OTHER THAN YERSINIA PESTIS

Isolation

Identification

Feces, urine, rectal swabs

Rectal swabs

Agar slant: heart infusion, trypticase soy, blood or chocolate agar slant.

#219

Enteric pathogen kit with buffered glycerol saline. By request only. Mark slip “Yersinia”.

Enteric pathogen special kit with Cary-Blair preservative. Must be sent refrigerated. DO NOT FREEZE. Mark slip “Yersinia”.

Mailing container furnished by submitter.

Bacteriology

LAB 353

If you need any tests which are not listed in the above reference, please contact our Laboratories at 502/564-4446.

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DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

SHIGELLA	Isolation Identification (referred culture)	Feces, urine, rectal swabs Rectal Swabs Food Agar slant: Heart infustion, trypticase soy, blood or choco-late agar slant.	#219 #219 #504 #219	Enteric pathogens kit with buffered glycerol saline. Special kit with Cary-Blair preservative and cold packs. Must be sent refrigerated. DO NOT FREEZE. Mark slip “Shigella”. Refer to Manual for Environmentalists and Milk Inspectors Mailing container furnished by submitter.	Bacteriology LAB 353
SICKLE CELL (infant)	Electrophoresis	Heel-stick blood applied to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional information, see back of form.	Newborn Screening
SICKLE CELL (children & adults)	Electrophoresis	Fingerstick blood applied to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional information, see back of form.	Newborn Screening
SPOROTRICHOSIS	Serology	5 ml clotted blood or serum	#213	Serology container.	Serology
STAPHYLOCOCCUS AUREUS	Isolation	Feces, vomitus Nasal swabs, skin, skin lesions Food	#219 #219 #504	Enteric pathogens kit with buffered glycerol saline. Only by request of the Division of Epidemiology. Swabs in sterile tubes. Only by request of the Division of Epidemiology. Refer to Manual for Environmentalists and Milk Inspectors. Only by request of the Division of Epidemiology for foodbourne disease outbreaks.	Bacteriology LAB 353
DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

SYPHILIS Note:Screening and confirmation tests performed according to DLS established algarithm	VDRL Qualitative & Quantitative	Clotted blood, serum, or spinal fluid. Serum	#213	Syphilis serology kit.	Serology LAB 351
TAPEWORM	Macroscopic & microscopic exam, formalin Hemo-D concentrations.	Feces and/or worm. For feces, submit at least three specimens on alternate days.	#219	Intestinal parasites, single vial container (10% formalin); Sterile jar with 10% formalin or 70% ethyl alcohol for the worm.	Bacteriology LAB 349A
TRICHINOSIS	Microscopic exam Bentonite flocculation	Biopsy of muscle (for food, see “Manual for the Sanitarian”) 5ml whole blood or serum	#219 #213	Intestinal parasites single vial container (10% formalin) Serology container	Bacteriology LAB 349A
TRIGLYCERIDE		See Lipid Profile
TUBERCULOSIS	Microscopic exam, culture identification by DNA Probe or HPLC, drug susceptibility studies.	Sputum, gastric wash, spinal fluid, pleural fluid, pus, urine, other body fluids. Gastric wash must be neutralized before mailing. Referred culture for identification and drug suscept-ibility studies.	#207	TB specimen kit Mailing container furnished by submitter.	Mycobacteria Lab LAB 355
TULAREMIA Note: Call the Division of Epidemiology or the Division of Lab Service for specific instructions	Agglutination test Isolation Identification (Referred culture)	Acute and convalescent sera. Chocolate agar slant.	#213 #219	Serology container. Mailing container furnished by submitter.	Bacteriology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

TYPHOID SALMONELLA SEROTYPE TYPHI	Culture Isolation Antigenic typing	Feces, urine Feces, urine, rectal swab Rectal swabs Culture agar or slant without sugar.	#219 #219 #219	Enteric pathogens kit. Enteric pathogens basic kit with buffered glycerol saline. Enteric pathogens special kit with Cary-Blair preservative. Must be refrigerated. DO NOT FREEZE. Mark slip “Salmonella typhi”. Mailing container furnished by submitter.	Bacteriology LAB 353
UNDULANT FEVER		See Brucellosis
VARICELLA ZOSTER		See Chickenpox
VDRL		See Syphilis
VIBRIO CHOLERAE AND OTHER VIBRIO SPECIES	Isolation Identification (referred culture)	Feces, urine, rectal swabs Agar slant: heart infusion, trypticase soy, chocolate or blood agar slant.	#219 #219	Enteric pathogens special kit with Cary-Blair preservative. Must be refrigerated. DO NOT FREEZE. Mark slip “Vibrio”. Mailing container furnished by submitter.	Bacteriology LAB 353

NOTE: For bacteriological and Chemical water analyses, all samples must be collected and submitted by authorized collectors. All samples from private sources must be received within 48 hours of collection. All samples from public drinking water systems must be received within 30 hours of collection. If additional tests are needed, contact the Division of Laboratory Services.
DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

WATER: BACTERIO-LOGICAL ANALYSES	Fecal coliform count & total coliform count. Heterotrophic plate count Pseudomonas Total coliform count and E. coli count. Presence or absence of total coliforms & E. coli.	Recreational water. Recreational water. Water from spas. Drinking water. Water from dairies.	#507 #507 #507 #507	Refer to the Manual for Environmentalists & Milk Inspectors for all tests.	Sanitary Bacteriology LAB 507E
WATER: Chemical ANALYSES	Alkalinity Calcium Chloride Color Fluoride Hardness Iron Magnesium Manganese Nitrates Odor PH Solids Sulfate Turbidity Dental Fluoride Metals Pesticides Petroleum Products	Drinking water from private supplies.	#700b #505c #504 #504 #504	Chemical analysis cubitainer. Call CHS-DPH Maternal and Child Health. Metal Cubitainer Call the lab. Call the lab.	Instrumentation Chemistry
WHOOPING COUGH		See Bordetella
YERSINIA PESTIS (PLAGUE)	NOTE: Call the Division of Epidemiology or the Division of Laboratory Services for specific instructions before collecting any specimens.

DISEASE/

CONDITION

TEST

SPECIMEN

LAB FORM

CONTAINER

SECTION AND LABEL

YERSINIA SPECIES OTHER THAN YERSINIA PESTIS

Isolation

Identification

Feces, urine, rectal swabs

Rectal swabs

Agar slant: heart infusion, trypticase soy, blood or chocolate agar slant.

#219

Enteric pathogen kit with buffered glycerol saline. By request only. Mark slip “Yersinia”.

Enteric pathogen special kit with Cary-Blair preservative. Must be sent refrigerated. DO NOT FREEZE. Mark slip “Yersinia”.

Mailing container furnished by submitter.

Bacteriology

LAB 353

If you need any tests which are not listed in the above reference, please contact our Laboratories at 502/564-4446.

REFERENCE LIST OF LABORATORY TESTS CABINET FOR HEALTH SERVICES DEPARTMENT FOR PUBLIC HEALTH DIVISION OF LABORATORY SERVICES

DISEASE/

TEST

SPECIMEN

LAB FORM

CONTAINER

SHIGELLA

Isolation

Bacteriology

LAB 353

SICKLE CELL (infant)

Electrophoresis

Newborn Screening

SICKLE CELL (children & adults)

Electrophoresis

Newborn Screening

SPOROTRICHOSIS

Serology

Serology

STAPHYLOCOCCUS AUREUS

Isolation

Bacteriology

LAB 353

DISEASE/

TEST

SPECIMEN

LAB FORM

CONTAINER

SYPHILIS

VDRL Qualitative & Quantitative

Clotted blood, serum, or spinal fluid.

#213

TAPEWORM

Macroscopic & microscopic exam, formalin Hemo-D concentrations.

Feces and/or worm. For feces, submit at least three specimens on alternate days.

#219

TRICHINOSIS

Microscopic exam

Biopsy of muscle (for food, see “Manual for the Sanitarian”)

#219

TRIGLYCERIDE

See Lipid Profile

TUBERCULOSIS

Microscopic exam, culture identification by DNA Probe or HPLC, drug susceptibility studies.

Sputum, gastric wash*, spinal fluid, pleural fluid, pus, urine, other body fluids.

#207

TULAREMIA

Agglutination test

Acute and convalescent sera.

#213

DISEASE/

TEST

SPECIMEN

LAB FORM

CONTAINER

TYPHOID

Culture

Feces, urine

#219

Bacteriology

UNDULANT FEVER

See Brucellosis

VARICELLA ZOSTER

See Chickenpox

VDRL

See Syphilis

VIBRIO

Isolation

Feces, urine, rectal swabs

#219

Bacteriology

DISEASE/

TEST

SPECIMEN

LAB FORM

CONTAINER

WATER: BACTERIO-LOGICAL ANALYSES

Fecal coliform count & total coliform count.

Recreational water.

#507