REFERENCE LIST OF LABORATORY TESTS CABINET FOR HEALTH SERVICES DEPARTMENT FOR PUBLIC HEALTH DIVISION OF LABORATORY SERVICES

REFERENCE LIST OF LABORATORY TESTS

CABINET FOR HEALTH SERVICES

DEPARTMENT FOR PUBLIC HEALTH

DIVISION OF LABORATORY SERVICES

Telephone: 502/564-4446

The following is an alphabetical list of agents or conditions for which testing is available for county health departments. The specimen required for each condition, applicable mailing containers and submission forms are listed.

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

ADENOVIRUS	Isolation	Throat wash, throat swab, eye swab, rectal swab.	#275	Virus isolation swab collection kit.	Virology Label sent with kit
AIDS		See HIV-I
AMEBIASIS	Formalin-Hemo D concentrations. Microscopic exam. Trichrome stained slides.	Feces. At least three specimens collected on alternate days.	#219	Intestinal parasites, Two vials – PVA & 10% formalin.	Bacteriology LAB 349A
ANTHRAX NOTE: Call the Division of Epidemiology or the Division of Laboratory Services for specific instructions before collecting any specimen.	Isolation Identification (referred culture)	Agar slant: Trypticase soy, blood or chocolate slants	#219	Mailing container furnished by submitter.	Bacteriology
ASEPTIC MENINGITIS	Isolation	Feces/rectal swab, throat wash/swab, spinal fluid, autopsy tissue.	#275	Virus isolation swab-collection kit.	Virology
BACILLUS ANTHRACIS		See Anthrax
BACILLUS CEREUS	Isolation	Feces, urine, rectal swabs, vomitus.	#219	Enteric Pathogens complete kit with Cary-Blair preservative. Must be refrigerated.	Bacteriology LAB 353
	Food	NOTE: By request of Epidemiology ONLY in foodborne disease outbreaks.	#504 Mark form “Bacillus cereus”
	Identification (Referred Culture)	Agar slant: heart infusion, trypticase soy, blood or chocolate agar slant.	#219	Mailing container furnished by submitter.	Bacteriology
BALANTIDIASIS, BALANTIDIUM COLI	Formalin-Hemo D concentrations & microscopic examination. Microscopic exam of stained films.	Feces. At least three specimens collected on alternate days.	#219	Intestinal parasites, two vials – PVA & 10% formalin.	Bacteriology LAB 349A
DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

BORELLIA BURGDORFERI		See Lyme’s Disease
BORDETELLA PERTUSSIS (WHOOPING COUGH)	Isolation Identification (referred culture) Direct FA (agent detection)	Call Laboratory Services for specific instructions. Charcoal Blood Media,Bordet-Gengou plate or Jones-Kendick medium slant. Nasopharyngeal smears on 2 slides.	#219 #275	Mailing container furnished by submitter. Pertussis FA slide kit.	Bacteriology Virology
BOTULISM, CLOSTRIDIUM BOTULINUM	Patient’s physician must consult the Division of Epidemiology to verify the need to perform appropriate tests.	Consult with Laboratory Services for specific information on collecting and submitting specimens.
BRUCELLA BRUCELLOSIS Note: Call the Division of Epidemiology or the Division of Lab Services before collection	Isolation Identification (referred culture)	Trypticase soy agar slant.	#219	Mailing container furnished by submitter.	Bacteriology
BUBONIC PLAGUE		See Yersinia Pestis
BURKHOLDRIA MALLEI		See Glander’s
CAMPYLOBACTER	Isolation Identification (referred culture)	Feces, rectal swabs, swabs dipped in feces. Swab from culture in Cary Blair, Campy thio or campy blood plate in bag with Campy gas or in semi-solid motility type medium.	#219 #219	Complete enteric pathogens kit with Cary-Blair. MUST be sent refrigerated. DO NOT FREEZE. Mark slip “Campylobacter” Mailing container furnished by submitter.	Bacteriology LAB 353 Bacteriology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

CHICKENPOX (VARICELLA-ZOSTER)	Isolation	Vesical fluid/swab	#275	Virus isolation swab-collection kit.	Virology LAB 356
CHICKENPOX (VARICELLA-ZOSTER)	Serology : EIA	Single serum (for immune status. Acute and convalescent sera (for diagnosis)	#275	Viral and rickettsial serology kit.	Virology LAB 356
CHLAMYDIA TRACHOMATIS	Amplified Nucleic Acid Probe	Urogenital & urine	#194	Aptima specimen collection kits. NOTE: Available only through local health department prenatal, family planning clinics, and STD clinics.	Serology LAB 194A
CHOLERA		See Vibrio Cholera
CLOSTRIDIUM BOTULINUM		See Botulism	#230
CONGENITAL HYPO-THYROIDISM	FIA	Heel-stick blood applied to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional instructions, see back of form.
COXSACKIE VIRUS		See Enterovirus
CRYPTOCOCCOSIS	Serology	Sera, 5-8 ml clotted blood, or 1-2 ml spinal fluid.	#213		Serology LAB 351
CRYPTOSPORIDIUM	Microscopic exam of stained slides.	Feces, at least three specimens collected on alternate days.	#219	Intestinal parasites, single vial (10% formalin). Request cryptosporium exam.	Bacteriology LAB 349A
CYTOMEGA-LOVIRUS (CMV)	Isolation Serology : EIA	Fresh urine, deliver to Lab without delay, keep cold but do not freeze. Acute and convalescent sera.	#275 #275	Mailing container furnished by sender. Viral & rickettsial serology kit.	Virology
DIPHTHERIA	Isolation Identification (referred culture)	NOT AVAILABLE Prefer Loeffler’s or Pai slant. May use trypticase soy or heart infusion agar slant	#219	Mailing container furnished by submitter	Bacteriology
DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

DIARRHEA	Bacterial culture Viral Isolation	SEE Salmonella, Shigella, Campy-lobacter, Vibrio, Escherichia coli 0157:H7, Yersinia, Amebiasis, and Cryptosporidium Feces/rectal swab.	#275	Virus isolation swab collection kit.	Bacteriology LAB 353
DIPHTHERIA	Isolation Identification (referred culture)	NOT AVAILABLE Prefer Loeffler’s or Pai slant. May use trypticase soy or heart infusion agar slant.	#219	Mailing container furnished by submitter.
DYSENTERY (AMEBIC)		See Amebiasis.
ECHO VIRUS		See Enterovirus.
ENCEPHALITIS VIRAL Encephalitis ARBO Viral St. Louis Eastern Equine Western Equine California Grp West Nile Virus	Isolation (other than ARBO) Serology : EIA	Spinal fluid, feces/rectal swab, throat wash/swab, autopsy tissue. Acute and convalescent sera. Spinal Fluid	#275 #275	Virus isolation swab collection kit. Viral & rickettsial serology kit.	Virology
ENTERO-BIASIS (PINWORMS)	Direct Examination	Follow kit instructions. Submit specimens on three consecutive days.	#219	Pinworm tube outfit.	Bacteriology LAB 349A
ENTERO-HEMORRHAGIC E. COLI (EHEC)	EIA	E.Coli on agar slant: Trypticase Soy, blood or chocolate slant	#219	Mailing container furnished by submitter	Bacteriology
ENTEROVIRUS (Coxsackie, ECHO, Reovirus, Polio)	Isolation	Feces/rectal swab, spinal fluid, throat wash/swab, autopsy tissue.	#275	Virus isolation swab collection kit.	Virology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

ESCHERICHIA COLI O157:H7	Isolation Identification	Feces Rectal Swabs Rectal Swabs 10 picks from MacConkey sent on separate agar slants without sugar, or 5 picks of colorless colonies from MacConkey D-Sorbitol agar.	#219 #219 #219	Enteric pathogens kit with buffered glycerol saline. Special kit with Cary-Blair preservative and cold packs. Must be refrigerated. DO NOT FREEZE. Mark slip “Escherichia coli O157:H7”. Mailing container furnished by submitter.	Bacteriology LAB 353
FLUORESCENT TREPONEMA ANTIBODY (FTA)	For diagnostic problem cases only.	See Syphilis
FRANCISELLA TULARENSIS		See Tularemia
FOODS: Chemical ANALYSIS	Possible contamination, adulteration, mislabeling, preservatives, & decomposition of foods.	Packaged food.	#504	Available only through county environmentalists.
FOOD AND WATERBORNE DISEASES	Bacteriological exam of milk/ Dairy products. Water bacteriology. Foodborne disease investigations.			Refer to Manual for Environmentalists & Milk Inspectors for all tests.
GALACTOSEMIA	Beutler-Baluda	Heel-stick blood applies to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional instructions, see back of form.	Newborn Screening
GIARDIASIS	Formalin-Hemo-D concentration and microscopic exam of trichrome stained slides.	Feces. At least three specimens collected on alternate days.	#219	Intestinal parasites two vial container (PVA & 10% formalin)	Bacteriology LAB 349A

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

GLANDERS DISEASE	Identification	TSA, blood, or chocolate agar slant.	#219	Mailing container furnished by submitter.	Bacteriology
GLUCOSE	1 hour post 50 gram load.	Plasma, 7ml whole blood in gray stoppered tube.	#230	GLUCOSE	Clinical Chemistry LAB 305
GLUCOSE FASTING or RANDOM		Plasma, 7ml whole blood in gray stoppered tube.	#230		Clinical Chemistry LAB 305
GLUCOSE TOLERANCE	Done only for Pre & Post Natal Patients.	7 ml whole blood in gray-stoppered tube.	#230	GLUCOSE TOLERANCE	Clinical Chemistry LAB 305
GONORRHOEA	Amplified Nucleic Acid Probe Smear for gram stain.	Urogenital swab and urine MALE: Anogenital sources ONLY. FEMALE: Direct smear not available.	#194 #219	Aptima specimen collection kits. Gonorrhea slide kit.	Serology LAB 194A
NEISSERIA GONORRHOEA	Amplified PCR (Note: Available only through local health department, prenatal, family planning, clinics and STD clinics.	Endocervical or urogenital swab or urine	#194	Aptima specimen collection kits.	Serology LAB 194A
NEISSERIA GONORRHOEA & OTHER NEISSERIA SPECIES	Isolation Identification (referred culture) NOTE: All N. gonorrhoeae isolates are tested for beta-lactamase, penicillin, & tetracycline susceptibility. N. meningitidis isolates are grouped.	Not available. Suspected N. gonorrhoeae, N. lactamica or N. meningitidis use Martin Lewis or chocolate plate in bag with C02 pill. Other species, use chocolate agar plate or slant. Specimen must be received within 72 hours of initial culturing or subculturing. Incubate subcultures 24 hours before shipping.	#219	GC culture container.	Serology
HAEMOPHILUS INFLUENZA	Typing for b	Pure culture on chocolate agar.	#219	Mailing container furnished by submitter.	Virology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

HEPATITIS	HbsAg Anti-HBs Anti-HBc Anti-HAV	Serum, NOTE: Available only through local health department and prenatal clinics. Serum, NOTE: Available through Epidemiology	#213 #213	Hepatitis Serology kit. Hepatitis Serology kit.	Serology LAB 351
HERPES SIMPLEX	Isolation Antigen detection: DFA Serology : EIA	Vesicle Fluid, spinal fluid, throat wash swab, lesion swab. Skin lesion smears in saline on slide. Acute and convalescent sera.	#275 #275 #275	Virus isolation swab collection kit. Herpes FA slide kit. Viral & rickettsial serology kit.	Serology LAB 351
HERPES ZOSTER		See Chickenpox
HIV-I	EIA, Western Blot NOTE: Available only through counseling & testing sites or sexually transmitted disease clinics. Forms and numbered stickers available ONLY from S.T.D.	Serum	#197		Serology LAB 351
HYPOTHYROID		See Congenital Hypothyroidism
INFLUENZA	Isolation Serology :	Nasopharyngeal swab/Throat swab/wash. Acute and convalescent sera.	#275 #275	Virus isolation swab collection kit. Viral & rickettsial serology kit.	Virology Label Provided in kit
INTESTINAL HELMINTHS	Formalin-Hemo-D concentration & microscopic examination.	Feces or passed worm. For feces, submit at least three specimens on alternate days.	#219	Intestinal parasites, single vial container (10% formalin)	Bacteriology LAB 349A
INTESTINAL PARASITES		See Intestinal Helminths and Intestinal Protozoa.

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

INTESTINAL PROTOZOA	Formalin-Hemo-D concentration & microscopic examination. Trichrome Smears.	Feces. Submit at least three specimens on alternate days.	#219	Intestinal parasites, two vial container (PVA & 10% formalin).	Bacteriology LAB 349A
LEAD	For information on blood lead screening, call the Louisville-Jefferson County Health Department at 502/574-6530.
LEGIONNAIRES’ DISEASE	DFA (agent detection)	Urine	#275	Mailing container furnished by submitter.	Virology
LEPTOSPIROSIS	Agglutination	Acute and convalescent sera.	#213	Serology container.	Serology
LIPID PROFILE		Serum or whole blood in 7 ml red-stoppered tube.	#230		Clinical Chemistry LAB 305
LISTERIA MONO-CYTOGENES	DNA Probe	TSA, or blood agar slant.	#219	Mailing container furnished by submitter.	Bacteriology
LITHIUM LEVEL	Provided ONLY for Comprehensive care patients with doctor’s order.	Serum	#230		Clinical Chemisty LAB 305
LYME DISEASE	Serology (CDC)	Prior consul-tation with the Division of Epidemiology.	#275	Viral & rickettsial serology kit.	Virology
LYME’S DISEASE	PCR (CDC)	Tick	#219	Mailing container furnished by submitter.	Virology
MALARIA	Microscopic exam (CDC)	Thick & thin blood smears.	#219	Slide and slide mailers are provided by sender.	Virology
MEASLES		See Rubeola
MILK & DAIRY PRODUCTS	Refer to Manual for Environmentalists and Milk Inspectors.
MISCELANEOUS BACTERIOLOGY	Identification (referred culture)	Tubed media on which the organism grows best.	#219	Mailing container furnished by submitter. Service provided only for problem organisms.	Bacteriology
MUMPS	Serology : EIA	Single serum (for immune status) Acute and convalescent sera (for diagnosis)	#275		Virology LAB 356
MYCOBACTERIA		See Tuberculosis

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

MYOCARDITIS		See Enterovirus – Coxsackie
NEWBORN SCREENING TESTS FOR: Phenylkentonuria Galactosemia Congenital Hypothyrodism Sickle Cell	See individual disease	Heel-stick blood applied to filter paper. Dry before mailing.			Newborn Screening
OPTHALMIA NEONATORUM	Microscopic exam.	Smear of exudate.	#219	Gonorrhea slide kit.	Serology LAB 194A
PERICARDITIS		See Enterovirus- Coxsackie
PERTUSSIS		See BORDETELLA PERTUSSIS
PHENYL-KETONURIA (PKU)	EIA	Heel-stick blood applied to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional instructions, see back of form.	Newborn Screening
PINWORMS		See Enterobiasis
POLIOMYE-LITIS		See Enterovirus

PRENATAL PROFILE	VDRL HbsAg Rubella	Two full 7 ml clot tubes.	#212	Prenatal profile kit available through prenatal clinics or local health department.	Serology LAB 359 or LAB 351
RABIES IN ANIMALS	Microscopic exams: Isolation if human exposure case.	Animal Head (refrigerated)	#254	Rabies shipping container.	Virology
REOVIRUS		See Enterovirus
RESPIRATORY SYNCYTIAL VIRUS	Isolation	Throat wash/ Swab (Do not freeze, but keep cold.) NOTE: Rush, virus not stable.	#275	Virus isolation swab collection kit.	Virology Label sent with kit
RHEUMATIC FEVER		See Streptococcal Infection
ROCKY MOUNTAIN SPOTTED FEVER	Serology (CDC)	Acute and convalescent sera.	#275	Viral & rickettsial serology kit.	Virology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

RUBELLA (German Measles)	Serology: EIA: IgG EIA: IgM	Single serum (for immune status) Acute and convalescent sera (for diagnosis) Serum for diagnosis	#275 #213	NOTE: Prior approval from the Division of Epidemiology.	Serology LAB 351
RUBEOLA (MEASLES)	Serology: EIA: IgG EIA: IgM	Single serum (for immune status) Serum for diagnosis	#275	Viral & rickettsial serology kit.	Virology LAB 356
SALMONELLA (ALL SERO-TYPES)	Isolation Identification (referred culture)	Feces, urine, rectal swabs. Rectal Swabs Food Agar slant: heart infusion, trypticase soy, blood or chocolate agar slant.	#219 #219 #504 #219	Enteric pathogens kit with buffered glycerol saline preservatives. Special kit with Cary-Blair preservative and cold packs. Must be refrigerated. DO NOT FREEZE. Mark slip “Salmonella” Refer to the Manual for Environmentalists and Milk Inspectors. Mailing Container furnished by submitter.	Bacteriology LAB 353

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REFERENCE LIST OF LABORATORY TESTS

CABINET FOR HEALTH SERVICES

DEPARTMENT FOR PUBLIC HEALTH

DIVISION OF LABORATORY SERVICES

Telephone: 502/564-4446

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

ADENOVIRUS	Isolation	Throat wash, throat swab, eye swab, rectal swab.	#275	Virus isolation swab collection kit.	Virology Label sent with kit
AIDS		See HIV-I
AMEBIASIS	Formalin-Hemo D concentrations. Microscopic exam. Trichrome stained slides.	Feces. At least three specimens collected on alternate days.	#219	Intestinal parasites, Two vials – PVA & 10% formalin.	Bacteriology LAB 349A
ANTHRAX NOTE: Call the Division of Epidemiology or the Division of Laboratory Services for specific instructions before collecting any specimen.	Isolation Identification (referred culture)	Agar slant: Trypticase soy, blood or chocolate slants	#219	Mailing container furnished by submitter.	Bacteriology
ASEPTIC MENINGITIS	Isolation	Feces/rectal swab, throat wash/swab, spinal fluid, autopsy tissue.	#275	Virus isolation swab-collection kit.	Virology
BACILLUS ANTHRACIS		See Anthrax
BACILLUS CEREUS	Isolation	Feces, urine, rectal swabs, vomitus.	#219	Enteric Pathogens complete kit with Cary-Blair preservative. Must be refrigerated.	Bacteriology LAB 353
	Food	NOTE: By request of Epidemiology ONLY in foodborne disease outbreaks.	#504 Mark form “Bacillus cereus”
	Identification (Referred Culture)	Agar slant: heart infusion, trypticase soy, blood or chocolate agar slant.	#219	Mailing container furnished by submitter.	Bacteriology
BALANTIDIASIS, BALANTIDIUM COLI	Formalin-Hemo D concentrations & microscopic examination. Microscopic exam of stained films.	Feces. At least three specimens collected on alternate days.	#219	Intestinal parasites, two vials – PVA & 10% formalin.	Bacteriology LAB 349A
DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

BORELLIA BURGDORFERI		See Lyme’s Disease
BORDETELLA PERTUSSIS (WHOOPING COUGH)	Isolation Identification (referred culture) Direct FA (agent detection)	Call Laboratory Services for specific instructions. Charcoal Blood Media,Bordet-Gengou plate or Jones-Kendick medium slant. Nasopharyngeal smears on 2 slides.	#219 #275	Mailing container furnished by submitter. Pertussis FA slide kit.	Bacteriology Virology
BOTULISM, CLOSTRIDIUM BOTULINUM	Patient’s physician must consult the Division of Epidemiology to verify the need to perform appropriate tests.	Consult with Laboratory Services for specific information on collecting and submitting specimens.
BRUCELLA BRUCELLOSIS Note: Call the Division of Epidemiology or the Division of Lab Services before collection	Isolation Identification (referred culture)	Trypticase soy agar slant.	#219	Mailing container furnished by submitter.	Bacteriology
BUBONIC PLAGUE		See Yersinia Pestis
BURKHOLDRIA MALLEI		See Glander’s
CAMPYLOBACTER	Isolation Identification (referred culture)	Feces, rectal swabs, swabs dipped in feces. Swab from culture in Cary Blair, Campy thio or campy blood plate in bag with Campy gas or in semi-solid motility type medium.	#219 #219	Complete enteric pathogens kit with Cary-Blair. MUST be sent refrigerated. DO NOT FREEZE. Mark slip “Campylobacter” Mailing container furnished by submitter.	Bacteriology LAB 353 Bacteriology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

CHICKENPOX (VARICELLA-ZOSTER)	Isolation	Vesical fluid/swab	#275	Virus isolation swab-collection kit.	Virology LAB 356
CHICKENPOX (VARICELLA-ZOSTER)	Serology : EIA	Single serum (for immune status. Acute and convalescent sera (for diagnosis)	#275	Viral and rickettsial serology kit.	Virology LAB 356
CHLAMYDIA TRACHOMATIS	Amplified Nucleic Acid Probe	Urogenital & urine	#194	Aptima specimen collection kits. NOTE: Available only through local health department prenatal, family planning clinics, and STD clinics.	Serology LAB 194A
CHOLERA		See Vibrio Cholera
CLOSTRIDIUM BOTULINUM		See Botulism	#230
CONGENITAL HYPO-THYROIDISM	FIA	Heel-stick blood applied to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional instructions, see back of form.
COXSACKIE VIRUS		See Enterovirus
CRYPTOCOCCOSIS	Serology	Sera, 5-8 ml clotted blood, or 1-2 ml spinal fluid.	#213		Serology LAB 351
CRYPTOSPORIDIUM	Microscopic exam of stained slides.	Feces, at least three specimens collected on alternate days.	#219	Intestinal parasites, single vial (10% formalin). Request cryptosporium exam.	Bacteriology LAB 349A
CYTOMEGA-LOVIRUS (CMV)	Isolation Serology : EIA	Fresh urine, deliver to Lab without delay, keep cold but do not freeze. Acute and convalescent sera.	#275 #275	Mailing container furnished by sender. Viral & rickettsial serology kit.	Virology
DIPHTHERIA	Isolation Identification (referred culture)	NOT AVAILABLE Prefer Loeffler’s or Pai slant. May use trypticase soy or heart infusion agar slant	#219	Mailing container furnished by submitter	Bacteriology
DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

DIARRHEA	Bacterial culture Viral Isolation	SEE Salmonella, Shigella, Campy-lobacter, Vibrio, Escherichia coli 0157:H7, Yersinia, Amebiasis, and Cryptosporidium Feces/rectal swab.	#275	Virus isolation swab collection kit.	Bacteriology LAB 353
DIPHTHERIA	Isolation Identification (referred culture)	NOT AVAILABLE Prefer Loeffler’s or Pai slant. May use trypticase soy or heart infusion agar slant.	#219	Mailing container furnished by submitter.
DYSENTERY (AMEBIC)		See Amebiasis.
ECHO VIRUS		See Enterovirus.
ENCEPHALITIS VIRAL Encephalitis ARBO Viral St. Louis Eastern Equine Western Equine California Grp West Nile Virus	Isolation (other than ARBO) Serology : EIA	Spinal fluid, feces/rectal swab, throat wash/swab, autopsy tissue. Acute and convalescent sera. Spinal Fluid	#275 #275	Virus isolation swab collection kit. Viral & rickettsial serology kit.	Virology
ENTERO-BIASIS (PINWORMS)	Direct Examination	Follow kit instructions. Submit specimens on three consecutive days.	#219	Pinworm tube outfit.	Bacteriology LAB 349A
ENTERO-HEMORRHAGIC E. COLI (EHEC)	EIA	E.Coli on agar slant: Trypticase Soy, blood or chocolate slant	#219	Mailing container furnished by submitter	Bacteriology
ENTEROVIRUS (Coxsackie, ECHO, Reovirus, Polio)	Isolation	Feces/rectal swab, spinal fluid, throat wash/swab, autopsy tissue.	#275	Virus isolation swab collection kit.	Virology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

ESCHERICHIA COLI O157:H7	Isolation Identification	Feces Rectal Swabs Rectal Swabs 10 picks from MacConkey sent on separate agar slants without sugar, or 5 picks of colorless colonies from MacConkey D-Sorbitol agar.	#219 #219 #219	Enteric pathogens kit with buffered glycerol saline. Special kit with Cary-Blair preservative and cold packs. Must be refrigerated. DO NOT FREEZE. Mark slip “Escherichia coli O157:H7”. Mailing container furnished by submitter.	Bacteriology LAB 353
FLUORESCENT TREPONEMA ANTIBODY (FTA)	For diagnostic problem cases only.	See Syphilis
FRANCISELLA TULARENSIS		See Tularemia
FOODS: Chemical ANALYSIS	Possible contamination, adulteration, mislabeling, preservatives, & decomposition of foods.	Packaged food.	#504	Available only through county environmentalists.
FOOD AND WATERBORNE DISEASES	Bacteriological exam of milk/ Dairy products. Water bacteriology. Foodborne disease investigations.			Refer to Manual for Environmentalists & Milk Inspectors for all tests.
GALACTOSEMIA	Beutler-Baluda	Heel-stick blood applies to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional instructions, see back of form.	Newborn Screening
GIARDIASIS	Formalin-Hemo-D concentration and microscopic exam of trichrome stained slides.	Feces. At least three specimens collected on alternate days.	#219	Intestinal parasites two vial container (PVA & 10% formalin)	Bacteriology LAB 349A

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

GLANDERS DISEASE	Identification	TSA, blood, or chocolate agar slant.	#219	Mailing container furnished by submitter.	Bacteriology
GLUCOSE	1 hour post 50 gram load.	Plasma, 7ml whole blood in gray stoppered tube.	#230	GLUCOSE	Clinical Chemistry LAB 305
GLUCOSE FASTING or RANDOM		Plasma, 7ml whole blood in gray stoppered tube.	#230		Clinical Chemistry LAB 305
GLUCOSE TOLERANCE	Done only for Pre & Post Natal Patients.	7 ml whole blood in gray-stoppered tube.	#230	GLUCOSE TOLERANCE	Clinical Chemistry LAB 305
GONORRHOEA	Amplified Nucleic Acid Probe Smear for gram stain.	Urogenital swab and urine MALE: Anogenital sources ONLY. FEMALE: Direct smear not available.	#194 #219	Aptima specimen collection kits. Gonorrhea slide kit.	Serology LAB 194A
NEISSERIA GONORRHOEA	Amplified PCR (Note: Available only through local health department, prenatal, family planning, clinics and STD clinics.	Endocervical or urogenital swab or urine	#194	Aptima specimen collection kits.	Serology LAB 194A
NEISSERIA GONORRHOEA & OTHER NEISSERIA SPECIES	Isolation Identification (referred culture) NOTE: All N. gonorrhoeae isolates are tested for beta-lactamase, penicillin, & tetracycline susceptibility. N. meningitidis isolates are grouped.	Not available. Suspected N. gonorrhoeae, N. lactamica or N. meningitidis use Martin Lewis or chocolate plate in bag with C02 pill. Other species, use chocolate agar plate or slant. Specimen must be received within 72 hours of initial culturing or subculturing. Incubate subcultures 24 hours before shipping.	#219	GC culture container.	Serology
HAEMOPHILUS INFLUENZA	Typing for b	Pure culture on chocolate agar.	#219	Mailing container furnished by submitter.	Virology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

HEPATITIS	HbsAg Anti-HBs Anti-HBc Anti-HAV	Serum, NOTE: Available only through local health department and prenatal clinics. Serum, NOTE: Available through Epidemiology	#213 #213	Hepatitis Serology kit. Hepatitis Serology kit.	Serology LAB 351
HERPES SIMPLEX	Isolation Antigen detection: DFA Serology : EIA	Vesicle Fluid, spinal fluid, throat wash swab, lesion swab. Skin lesion smears in saline on slide. Acute and convalescent sera.	#275 #275 #275	Virus isolation swab collection kit. Herpes FA slide kit. Viral & rickettsial serology kit.	Serology LAB 351
HERPES ZOSTER		See Chickenpox
HIV-I	EIA, Western Blot NOTE: Available only through counseling & testing sites or sexually transmitted disease clinics. Forms and numbered stickers available ONLY from S.T.D.	Serum	#197		Serology LAB 351
HYPOTHYROID		See Congenital Hypothyroidism
INFLUENZA	Isolation Serology :	Nasopharyngeal swab/Throat swab/wash. Acute and convalescent sera.	#275 #275	Virus isolation swab collection kit. Viral & rickettsial serology kit.	Virology Label Provided in kit
INTESTINAL HELMINTHS	Formalin-Hemo-D concentration & microscopic examination.	Feces or passed worm. For feces, submit at least three specimens on alternate days.	#219	Intestinal parasites, single vial container (10% formalin)	Bacteriology LAB 349A
INTESTINAL PARASITES		See Intestinal Helminths and Intestinal Protozoa.

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

INTESTINAL PROTOZOA	Formalin-Hemo-D concentration & microscopic examination. Trichrome Smears.	Feces. Submit at least three specimens on alternate days.	#219	Intestinal parasites, two vial container (PVA & 10% formalin).	Bacteriology LAB 349A
LEAD	For information on blood lead screening, call the Louisville-Jefferson County Health Department at 502/574-6530.
LEGIONNAIRES’ DISEASE	DFA (agent detection)	Urine	#275	Mailing container furnished by submitter.	Virology
LEPTOSPIROSIS	Agglutination	Acute and convalescent sera.	#213	Serology container.	Serology
LIPID PROFILE		Serum or whole blood in 7 ml red-stoppered tube.	#230		Clinical Chemistry LAB 305
LISTERIA MONO-CYTOGENES	DNA Probe	TSA, or blood agar slant.	#219	Mailing container furnished by submitter.	Bacteriology
LITHIUM LEVEL	Provided ONLY for Comprehensive care patients with doctor’s order.	Serum	#230		Clinical Chemisty LAB 305
LYME DISEASE	Serology (CDC)	Prior consul-tation with the Division of Epidemiology.	#275	Viral & rickettsial serology kit.	Virology
LYME’S DISEASE	PCR (CDC)	Tick	#219	Mailing container furnished by submitter.	Virology
MALARIA	Microscopic exam (CDC)	Thick & thin blood smears.	#219	Slide and slide mailers are provided by sender.	Virology
MEASLES		See Rubeola
MILK & DAIRY PRODUCTS	Refer to Manual for Environmentalists and Milk Inspectors.
MISCELANEOUS BACTERIOLOGY	Identification (referred culture)	Tubed media on which the organism grows best.	#219	Mailing container furnished by submitter. Service provided only for problem organisms.	Bacteriology
MUMPS	Serology : EIA	Single serum (for immune status) Acute and convalescent sera (for diagnosis)	#275		Virology LAB 356
MYCOBACTERIA		See Tuberculosis

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

MYOCARDITIS		See Enterovirus – Coxsackie
NEWBORN SCREENING TESTS FOR: Phenylkentonuria Galactosemia Congenital Hypothyrodism Sickle Cell	See individual disease	Heel-stick blood applied to filter paper. Dry before mailing.			Newborn Screening
OPTHALMIA NEONATORUM	Microscopic exam.	Smear of exudate.	#219	Gonorrhea slide kit.	Serology LAB 194A
PERICARDITIS		See Enterovirus- Coxsackie
PERTUSSIS		See BORDETELLA PERTUSSIS
PHENYL-KETONURIA (PKU)	EIA	Heel-stick blood applied to filter paper. Dry before mailing.	#228	Mail in envelope. Do not fold. Do not place in plastic bag. For additional instructions, see back of form.	Newborn Screening
PINWORMS		See Enterobiasis
POLIOMYE-LITIS		See Enterovirus

PRENATAL PROFILE	VDRL HbsAg Rubella	Two full 7 ml clot tubes.	#212	Prenatal profile kit available through prenatal clinics or local health department.	Serology LAB 359 or LAB 351
RABIES IN ANIMALS	Microscopic exams: Isolation if human exposure case.	Animal Head (refrigerated)	#254	Rabies shipping container.	Virology
REOVIRUS		See Enterovirus
RESPIRATORY SYNCYTIAL VIRUS	Isolation	Throat wash/ Swab (Do not freeze, but keep cold.) NOTE: Rush, virus not stable.	#275	Virus isolation swab collection kit.	Virology Label sent with kit
RHEUMATIC FEVER		See Streptococcal Infection
ROCKY MOUNTAIN SPOTTED FEVER	Serology (CDC)	Acute and convalescent sera.	#275	Viral & rickettsial serology kit.	Virology

DISEASE/ CONDITION	TEST	SPECIMEN	LAB FORM	CONTAINER	SECTION AND LABEL

RUBELLA (German Measles)	Serology: EIA: IgG EIA: IgM	Single serum (for immune status) Acute and convalescent sera (for diagnosis) Serum for diagnosis	#275 #213	NOTE: Prior approval from the Division of Epidemiology.	Serology LAB 351
RUBEOLA (MEASLES)	Serology: EIA: IgG EIA: IgM	Single serum (for immune status) Serum for diagnosis	#275	Viral & rickettsial serology kit.	Virology LAB 356
SALMONELLA (ALL SERO-TYPES)	Isolation Identification (referred culture)	Feces, urine, rectal swabs. Rectal Swabs Food Agar slant: heart infusion, trypticase soy, blood or chocolate agar slant.	#219 #219 #504 #219	Enteric pathogens kit with buffered glycerol saline preservatives. Special kit with Cary-Blair preservative and cold packs. Must be refrigerated. DO NOT FREEZE. Mark slip “Salmonella” Refer to the Manual for Environmentalists and Milk Inspectors. Mailing Container furnished by submitter.	Bacteriology LAB 353

REFERENCE LIST OF LABORATORY TESTS CABINET FOR HEALTH SERVICES DEPARTMENT FOR PUBLIC HEALTH DIVISION OF LABORATORY SERVICES

DISEASE/

TEST

SPECIMEN

LAB FORM

CONTAINER

ADENOVIRUS

AIDS

AMEBIASIS

ANTHRAX

ASEPTIC MENINGITIS

DISEASE/

TEST

SPECIMEN

LAB FORM

CONTAINER

BORELLIA BURGDORFERI

BORDETELLA PERTUSSIS

BOTULISM, CLOSTRIDIUM BOTULINUM

Patient’s physician must consult the Division of Epidemiology to verify the need to perform appropriate tests.

Consult with Laboratory Services for specific information on collecting and submitting specimens.

BRUCELLA BRUCELLOSIS

Isolation

BUBONIC PLAGUE

See Yersinia Pestis

BURKHOLDRIA MALLEI

See Glander’s

CAMPYLOBACTER

Isolation

Feces, rectal swabs, swabs dipped in feces.

TEST

SPECIMEN

LAB FORM

CONTAINER

Isolation

CHLAMYDIA TRACHOMATIS

Amplified Nucleic Acid Probe

Urogenital & urine

CHOLERA

See Vibrio Cholera

CLOSTRIDIUM BOTULINUM

See Botulism

CONGENITAL HYPO-THYROIDISM

Heel-stick blood applied to filter paper. Dry before mailing.

COXSACKIE VIRUS

See Enterovirus

CRYPTOCOCCOSIS

Serology

Sera, 5-8 ml clotted blood, or 1-2 ml spinal fluid.

CRYPTOSPORIDIUM

Microscopic exam of stained slides.

Feces, at least three specimens collected on alternate days.

CYTOMEGA-LOVIRUS (CMV)

Fresh urine, deliver to Lab without delay, keep cold but do not freeze.

DISEASE/

TEST

SPECIMEN

LAB FORM

CONTAINER

DIARRHEA

Bacterial culture

SEE Salmonella, Shigella, Campy-lobacter, Vibrio, Escherichia coli 0157:H7, Yersinia, Amebiasis, and Cryptosporidium

DIPHTHERIA

Isolation

NOT AVAILABLE

DYSENTERY (AMEBIC)

See Amebiasis.

ECHO VIRUS

See Enterovirus.

ENCEPHALITIS

Isolation (other than ARBO)

Spinal fluid, feces/rectal swab, throat wash/swab, autopsy tissue.

ENTERO-BIASIS (PINWORMS)

Direct Examination

Follow kit instructions. Submit specimens on three consecutive days.

ENTERO-HEMORRHAGIC E. COLI

EIA

E.Coli on agar slant: Trypticase Soy, blood or chocolate slant

ENTEROVIRUS (Coxsackie, ECHO, Reovirus, Polio)

Isolation