Seminar 
 
This year the National Veterinary Institute, Technical University of Denmark has achieved a fund from FOOD-DTU for an International Cooperation Program to promote the international cooperation in basic research. We would like to invite you to attend a seminar on

 
MicroRNA deregulation and function in lung cancer 
 
Speaker  
Dr. Bernard Pierre MARI  
Institut de Pharmacologie Moléculaire et Cellulaire (IPMC)  
Nice-Sophia Antipolis, France

 
Time and place  
Thursday 29 May 2008 at 11:00 am

 
Laboratory of Applied Micro and Nanotechnology  
Department of Poultry, Fish and Fur Animals  
National Veterinary Institute  
Hangøvej 2 DK8200 Aarhus N  
 
For further information contact Dang Duong Bang Tel: + 45 7234 6892 or Søren Peter Jonstrup Tel:23620112 or e-mail spjo@vet.dtu.dk  
 
Abstract:  
Micro RNAs (miRNA) are small non-coding RNAs regulating gene expression by hybridization to complementary sequences usually located in the 3´untranslated region of coding transcripts. Association of miRNA with an RNA-multiprotein complex (RISC-RNA induced silencing complex) results either in inhibition of mRNA translation or reduced mRNA stability. Recently, aberrant expression of specific miRNAs has been described in human malignancies such as leukemias, lymphomas, and various carcinomas including non small cell lung cancer. Accordingly, miRNA expression profiles can be used to classify human cancer and miRNA signatures have been correlated with clinical outcome including metastatic spreading and patient survival. In humans, conservative predictions indicate that up to 30% of protein-coding genes may be regulated by such a mechanism. Thus, potentially all the cellular pathways may be governed by miRNAs, which may contribute to the fine-tuning of gene expression on a global level.  
Our project aims at determining the deregulation of miRNA expression in lung cancer and their impact on cancer progression, with a special emphasis on their role in tumor-host interaction. Using our miR chip, we have found several differentially expressed miRNA in tumoral /versus/ normal paired samples of lung adenocarcinoma. We have focused our attention on two miRNA, mir-155 and mir-210, that have been found to be overexpressed in tumor samples. I will present our recent data concerning the potential function of these two miRNA, in particular through the investigation of their cellular source by in situ hybridization, the set up of inducible expression models and the identification of their mRNA targets by both /in silico/ and experimental approaches.  
 
Introduction to Dr. Bernard Pierre MARI  
Institut de Pharmacologie Moléculaire et Cellulaire (IPMC)  
Nice-Sophia Antipolis, France  
Dr. Bernard Pierre MARI is a senior scientist and head of group of “Physiological Genomics” directed by Dr Pascal Barbry at IPMC. His recent research focuses on epithelial-stromal interactions and tumor progression.  
He studied molecular biology and medicine and receiving his BSc in 1990, his PhD from the Medecine Pasteur, University of Nice, France in 1994. After four years (1995-1998) of postdoctoral research at the laboratory of Margaret Shipp at the Dana Farber Cancer Institute, Department of Adult Oncology, Boston MA, USA he was offered a permanent position at CNRS, chargé de recherché in 1999 and since 2001, he is group leader, INSERM U526, Nice, France (Director: Patrick Auberger, Ph.D). He was author or co-author of 49 peer reviewed international publications.  
_Selection of 5 publications over past 5 years  
_1. Fromigue, O., Louis, K., Dayem, M., Milanini, J., Pages, G., Tartare-Deckert, S., Ponzio, G., Hofman, P., Barbry, P., Auberger, P., and _Mari, B_. (2003) Gene expression profiling of normal human pulmonary fibroblasts following coculture with non-small-cell lung cancer cells reveals alterations related to matrix degradation, angiogenesis, cell growth and survival. /Oncogene/ 22, 8487-8497.  
2. Louis, K., Guerineau, N., Fromigue, O., Defamie, V., Collazos, A., Anglard, P., Shipp, M. A., Auberger, P., Joubert, D., and _Mari, B__._ (2005) Tumor cell-mediated induction of the stromal factor stromelysin-3 requires heterotypic cell contact-dependent activation of specific protein kinase C isoforms. /J Biol Chem/ 280, 1272-1283  
3. Triboulet, R., _Mari, B_., Lin, Y. L., Chable-Bessia, C., Bennasser, Y., Lebrigand, K., Cardinaud, B., Maurin, T., Barbry, P., Baillat, V., Reynes, J., Corbeau, P., Jeang, K. T., and Benkirane, M. (2007) Suppression of microRNA-silencing pathway by HIV-1 during virus replication. /Science/ 315, 1579-1582  
4. Pottier, N., Chupin, C., Defamie, V., Cardinaud, B., Sutherland, R., Rios, G., Gauthier, F., Wolters, P. J., Berthiaume, Y., Barbry, P., and _Mari, B_. (2007) Relationships between early inflammatory response to bleomycin and sensitivity to lung fibrosis: a role for dipeptidyl-peptidase I and tissue inhibitor of metalloproteinase-3? /Am J Respir Crit Care Med/ 176, 1098-1107  
5. Defamie, V., Laurens, M., Patrono, D., Devel, L., Brault, A., Saint-Paul, M. C., Yiotakis, A., Barbry, P., Gugenheim, J., Crenesse, D., Dive, V., Huet, P. M., and _Mari, B_. (2008) Matrix metalloproteinase inhibition protects rat livers from prolonged cold ischemia-warm reperfusion injury. /Hepatology/ 47, 177-185