原文

美国研究人员4月15日说，传统中国医学已使用数百年的针裂蹄木层孔菌可能具有阻止乳腺癌细胞生长的功效，有望成为抗击乳腺癌的新“武器”。美国研究人员所做的细胞实验显示，针裂蹄木层孔菌具有抗乳腺癌细胞的功效。研究人员认为，这种药材可能通过抑制“AKT”酶抗击乳腺癌细胞，“AKT”酶可控制促使细胞生长的“信号”。

路透社报道说，科学界此前已发现针裂蹄木层孔菌有抗皮肤癌、肺癌和前列腺癌的功效，但这项针对乳腺癌的新研究标志研究人员开始了解针裂蹄木层孔菌的抗癌机理。

美国印第安纳波利斯地区一个研究所的科研人员丹尼尔·斯利瓦说，实验中，针裂蹄木层孔菌提取物减慢了新生癌细胞的生长速度，阻止了向肿瘤提供养分的新生血管的产生。

斯利瓦说：“我们还没有能力把这些知识应用于当代医学……希望我们的研究将鼓励更多研究者探索如何把可入药的菌类应用于治疗癌症。”斯利瓦等人的研究成果刊登在《英国癌症杂志》（British Journal of Cancer）上。（来源：新华网）

文摘

（British Journal of Cancer），doi:10.1038/sj.bjc.6604319，D Sliva, V Slivova

Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling 
 
D Sliva1,2,3, A Jedinak1, J Kawasaki1, K Harvey1 and V Slivova1

 
1Cancer Research Laboratory, Methodist Research Institute, 1800 N Capitol Ave, E504, Indianapolis, IN 46202, USA 
 
2Department of Medicine, Indiana University, Indianapolis, IN, USA 
 
3Indiana University Simon Cancer Center, School of Medicine, Indiana University, Indianapolis, IN, USA

The antitumour activity of a medicinal mushroom Phellinus linteus (PL), through the stimulation of immune system or the induction of apoptosis, has been recently described. However, the molecular mechanisms responsible for the inhibition of invasive behaviour of cancer cells remain to be addressed. In the present study, we demonstrate that PL inhibits proliferation (anchorage-dependent growth) as well as colony formation (anchorage-independent growth) of highly invasive human breast cancer cells. The growth inhibition of MDA-MB-231 cells is mediated by the cell cycle arrest at S phase through the upregulation of p27Kip1 expression. Phellinus linteus also suppressed invasive behaviour of MDA-MB-231 cells by the inhibition of cell adhesion, cell migration and cell invasion through the suppression of secretion of urokinase-plasminogen activator from breast cancer cells. In addition, PL markedly inhibited the early event in angiogenesis, capillary morphogenesis of the human aortic endothelial cells, through the downregulation of secretion of vascular endothelial growth factor from MDA-MB-231 cells. These effects are mediated by the inhibition of serine-threonine kinase AKT signalling, because PL suppressed phosphorylation of AKT at Thr308 and Ser473 in breast cancer cells. Taken together, our study suggests potential therapeutic effect of PL against invasive breast cancer.